Transform Microscopy Data Into Stunning 3D Visualizations
Acto3D brings your fluorescence microscopy images to life with powerful real-time volume rendering technology, optimized specifically for Apple Silicon to deliver exceptional performance.
Advanced Visualization Tools
Acto3D delivers a comprehensive suite of specialized tools designed to explore complex volumetric data with unparalleled clarity and precision.
Real-Time Rendering
Experience fluid, interactive exploration of volumetric microscopy data with rendering optimized for Apple Silicon's powerful GPU capabilities.
Transfer Functions
Fine-tune color and opacity with intuitive controls for real-time feedback and perfect visualization of specific structures within your data.
Free Sectioning
Slice through your 3D data at any angle to reveal internal structures and relationships hidden in traditional 2D views.
3D Masking
Easily extract and isolate closed regions in 3D space—ideal for analyzing enclosed structures in complex samples.
Programmable Shaders
Create custom visualization pipelines with programmable Metal shaders, giving advanced users complete control over the rendering process.
Animation Creation
Generate compelling animations of your 3D data for presentations, publications, or educational materials with built-in animation tools.
Streamlined Workflow
From image acquisition to stunning visualization in five comprehensive steps
Acquire Microscopy Images
Capture your fluorescence microscopy images with your preferred imaging system. Acto3D supports 3D reconstruction from a wide range of fluorescence microscopy techniques, including light sheet, confocal and multiphoton microscopy.
Convert to TIFF with Fiji
Use Fiji (ImageJ) to process and convert your microscopy images to TIFF format, preparing them for 3D reconstruction and visualization.
Import Into Acto3D
Simply load your processed TIFF files into Acto3D to visualize them in 3D space. Instantly explore your data from multiple viewing angles for detailed inspection.
Fine-Tune Visualization
Use Acto3D's intuitive transfer function editor to perfect color and opacity across multiple channels, enhancing contrast and highlighting specific features within your complex volumetric data.
Create Dynamic Presentations
Acto3D enables immersive and interactive 3D visualizations that can be used across a variety of contexts—including presentations, publications, and education. Built-in animation tools further enhance your ability to communicate complex structures with compelling visual impact.
Advanced Programmable Features
Take complete control of your visualization with custom Metal shaders
01kernel void FTB( *param ) { 02 Cin = Cout; 03 Ain = Aout; 04 Cvoxel = (float4)args.tex.sample 05 (args.smp, texCoordinate); 06}
Unlike commercial software, Acto3D is fully open-source, letting you customize every aspect of the GPU rendering process. Advanced users gain full insight into how their microscopy data is visualized.
The key advantage of volume rendering is the ability to adjust visualization using transfer functions. Acto3D provides programmatic control over these functions for more detailed and precise tuning.
View DocumentationGet Acto3D Today
Free and open-source, optimized specifically for Apple Silicon Macs to deliver exceptional performance
